Inverse Regulation of Mir Let-7g and HMGA2 Gene in HPV-Infected and Not Infected Cervical Cancer Patients

Introduction: Cervical cancer is the fourth most common neoplasm among women worldwide, with the majority of cases occurring in developing countries. High-risk human papilloma viruses (HPVs) types are accounted for more than 90% of cervical carcinomas. Despite standard treatment for cervical cancer, no specific molecular markers can predict the clinical response of patients diagnosed yet. Decreased in the miRNAs Let-7 family expression is reported to be associated with cervical cancer and Re-expression of that inhibits the proliferation and migration of cells via HMGA2 in carcinoma. The aim of the current study was to determine the correlation of the miR Let-7g and HMGA2 gene expression in HPV-infected cervical cancer patients and compare it with not infected patients and control groups among participating refereed to MirzaKochak Khan Hospital, Tehran, Iran, 2015.

Material and Methods: Total of 60 samples including 20 HPV-infected cervical cancer, 20 patients with no HPV infection and 20 healthy individuals as control were collected. Cervical cancer was determined by Pap smear and tissue sampling tests. The HPV 16 and 18 types were investigated by Real Time High-Risk HPV assay. The RNA was extracted, quality and quantity of the RNA were determined and the expression of the Let-7g, HMGA2 and GAPDH genes were determined using Real-time quantitative PCR (qRT-PCR) and ∆∆CT computational techniques. Data were analyzed using Graphpad Prism 6 software.

Result: According to the results, miR Let-7g expression decreased in HPV infected and not infected cervical cancer patients compare to control group (P=0.0002, P=0.0132 respectively). We found a significant decrease in miR Let-7g expression in HPV infected compare to not infected patients (P=0.0001). We observed an increased HMGA2 gene expression in infected and not infected cervical cancer patients compared to healthy individuals (P=0.0001, P=0.0005 respectively). Also, a positive association was observed with increased in HMGA2 gene expression in HPV infected patients compare to not infected women (P=0.0001).

Conclusion: these results suggested miR Let-7g along with the HMGA2 gene is a useful indicator for early prognosis of the HPV infected cervical cancer.