Anti-angiogenic Potential of Mentha longifolia (Horse Mint): Chlorioallantoic Membrane Assay

Inhibition of angiogenesis is one of the mechanisms of action of anticancer drugs. The current study was designed to probe into anti-angiogenic effect of Mentha longifolia by employing Chorioallantoic membrane assay. White leghorn fertilized eggs were used in the study (n=10). On 6th day of incubation, aqueous solution of methanol extract (300-1000 µg/mL) of Mentha longifolia was applied to chicken embryos via CAM route. After 24 hours incubation, all eggs were opened carefully and CAM images were taken with camera. These images were loaded to SPIP software for quantification and assessment of structural changes in CAM. Blood vessels (primary, secondary and tertiary) diameters and CAM areas decreased in concentration dependent fashion in all treated groups as compared to values of control group. All embryos died at highest used concentration. It is concluded that methanol extract of Mentha longifolia possessed concentration dependent anti-angiogenic effect but toxic at 1000 µg/mL.